Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

MiR-206 inhibits reorganization of the cytoskeleton in melanoma cells by targeting DDX5

Shenglin Wu¹, Shan Nie², Jian Wang³

¹Department of Burn, Plastic Surgery, and Wound Repair, The People’s Hospital of LiShui, Lishui, Zhejiang Province 323000, China; ²Department of Stomatology, First Hospital of Nanchang City, Jiangxi Province 330006, China; ³Department of Dermatology, The First Affiliated Hospital of Nanchang University, Nanchang City, Jiangxi Province 330006, China.

For correspondence:- Jian Wang Email: jianw2570@163.com Tel:+867918692745

Accepted: 27 October 2021 Published: 30 November 2021

Citation: Wu S, Nie S, Wang J. MiR-206 inhibits reorganization of the cytoskeleton in melanoma cells by targeting DDX5. Trop J Pharm Res 2021; 20(11):2279-2285 doi: 10.4314/tjpr.v20i11.7

© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the role and mechanism of microRNA-206 (miR-206) in cytoskeleton reorganization in melanoma cells.

Methods: MiR-206 and RNA helicase p68 (DDX5) expression levels were measured in A375, A875, and HEM-M cells by quantitative real time polymerase chain reaction (qRT-PCR). A DDX5 overexpression cell line was constructed, and DDX5 overexpression, A375, and A875 cells were transfected with miR-206 mimic or DDX5 small interfering RNA (siRNA). Transwell assay was used to assess cell migration and invasion of A375 and A875 cells, while Luciferase reporter assay was used to determine the putative target of miR-206. DDX5, miR-206, vinculin, coronin3, and ezrin expression levels were evaluated by qRT-PCR. Protein expressions of DDX5, vinculin, coronin3, and ezrin were evaluated by western blot analysis.

Results: DDX5 expression was higher and miR-206 expression lower in A375 and A875 cells when compared to HEM-M cells (p < 0.05). Knockdown of DDX5 and overexpression of miR-206 repressed invasion and migration, and inhibited expression of vinculin, coronin3, and ezrin in A375 and A875 cells (p < 0.05). However, overexpression of DDX5 reversed the effect of miR-206 on cytoskeletal protein expression. Luciferase reporter assay data confirmed that DDX5 is a direct target of miR-206 (p < 0.05).

Conclusion: MiR-206 suppresses reorganization of the cytoskeleton in melanoma cells by targeting DDX5, and is thus, a promising target for the treatment of melanoma.

Keywords: Melanoma, MicroRNA-206, Cytoskeleton reorganization, RNA helicase p68